Before Associate Professor DNA amplicons (444 bps) were visualized on agarose gel. Cells that received no vaccine at all were used for control purposes.The researchers then investigated how different gene expressions in the cells changed over time. 5. doi: 10.1038/s41586-020-2639-4. Use this form if you have come across a typo, inaccuracy or would like to send an edit request for the content on this page. Differences in IgG Antibody Responses following BNT162b2 and mRNA-1273 SARS-CoV-2 Vaccines. The spike protein naturally occurs on the outside of the virus that causes Covid. At this stage, we do not know if DNA reverse transcribed from BNT162b2 is integrated into the cell genome. Bad Bunny. We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. By Meiling Lee. This paper compared protein sequences from the SARS-CoV-2 virus to protein sequences in humans and described where the latter are found in the body. MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. Imaging findings included inflammation, beading, stricturing, and dilation of the biliary tree. A new study from Lund University in Sweden on how the Pfizer-BioNTech COVID-19 vaccine affects human liver cells under experimental conditions, has been viewed more than 800,000 times in just over a week. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 Pfizers BNT162b2 mRNA Covid-19 vaccine was the first vaccine against the novel coronavirus (COVID-19) approved for distribution in the United States. 19.9M. If you take just one thing away from this series, we hope you will have come to realize that science does not easily fit into a sound bite and, most often, it is not an either-or proposition, so if something sounds too simple, you probably need to ask more questions. About this website World Health Organization Coronavirus (COVID-19) Dashboard. At the heart of the misconceptions about this paper is figure 1, which shows viral shedding among vaccinated (green) and unvaccinated (red) individuals. This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.5-2% of the injection site concentration. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Safety and Efficacy of the BNT162b2 mRNA COVID-19 Vaccine. AP'S ASSESSMENT: False. Philadelphia, PA 19104, Know My Rights About Surprise Medical Bills. They are not sure if it gets integrated in our DNA or not. It only presents the body with the instructions to build immunity.. and Terms of Use. Huh7 cells were treated, Immunohistochemistry of Huh7 cells treated, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. Available online: Mulligan M.J., Lyke K.E., Kitchin N., Absalon J., Gurtman A., Lockhart S., Neuzil K., Raabe V., Bailey R., Swanson K.A., et al. And, while this may be the case early during infection, there are a few additional considerations when thinking about these data: Hopefully these examples demonstrated some of the ways that a paper should be examined. BNT162b2 mRNA levels in Huh7 cells treated with BNT162b2. A new Swedish study published in MDPIfound that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact with your DNA in any way. The clip comes from a longer roundtable discussion that was posted March 8 by Christian broadcasting network Daystar, during which faith leaders discussed COVID-19 vaccines with the doctors. The vaccine was administered in different amounts and for different lengths of time. The results have been widely discussed across social mediabut the results have in many cases been misinterpreted. doi: 10.1056/NEJMoa2034577. Does the Pfizer-BioNTech mRNA vaccine get converted to DNA or not? Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. Moore also cautioned that the cells used in the study were quite different than primary cells in the body. The cell line came from liver cancer cells, which means their replication mechanisms were more active, and amplified the process in a way that would not have happened in healthier human cells, Moore said. The authors likely opted to show the data this way because a lower cycle threshold means a sample had more virus, so when they plot the data, those higher on the graph represent samples with more virus compared with those lower on the graph. (In-vitro Swedish Study)" by Drbeen Medical Lectures Thrombotic events have been reported as a rare side effect after ChAdox1nCOV-19 vaccination that may cause death of recipient. Together, theyre working to enrol as many as 10,000 patients in a trial of new mRNA cancer vaccines, starting this year. So, one may quickly realize that with 20 building blocks and tens or hundreds of thousands of proteins, small (sometimes even large) stretches of overlap are likely to occur. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rChapters00:00 introduction00:45 Link to the summary01:00 Review of the reference material02:19 Cell preparation for the vaccine03:35 Vaccine addition to the cells06:00 RT-PCR for find RNA and then DNA13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA18:27 Measuring the volume of proteins in the cytosol and the nucleus22:35 DNA purification and verification28:41 Results28:42 LNP enters the liver cells efficiently29:33 LINE-1 gene is expressed30:46 ORF-1 found in cytosol and the nucleus36:01 DNA from the vaccine found in the cell43:30 Questions not answeredDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. The Swedish study suggests the vaccine is capable of becoming DNA, which is what the CDC claimed In this case, the author compared gene sequences to identify sections of human proteins that have portions similar to SARS-CoV-2 proteins and made a prediction that the similarities would cause immune responses against oneself. 2021;385:759760. -, Walsh E.E., Frenck R.W., Jr., Falsey A.R., Kitchin N., Absalon J., Gurtman A., Lockhart S., Neuzil K., Mulligan M.J., Bailey R., et al. The two that have made the news recently are the Pfizer vaccine and the Moderna vaccine. The approval came as an emergency use authorization on December 11, 2020, after clinical trials showed the vaccine was 95% effective in preventing COVID-19. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage boys died in their sleep within the first week after receiving the 2nd Pfizer COVID-19 mRNA vaccine dose. Indeed, about 8 percent of human DNA comes from viruses inserted into our genomes during evolution. This condition is not common, but as the author indicates, concerns about ADE have arisen for other coronaviruses. By using our site, you acknowledge that you have read and understand our Privacy Policy The. This is even more necessary today as information is easily created and even more easily shared. Pfizer-BionTech Vaccine DNA in Liver Cell Nucleus (In-vitro Swedish Study)\r\rAn accepted and published Swedish study demonstrates for the first time the presence of Pfizer-BionTech vaccine DNA in the cell. What are the next steps in this research? Your email address is used only to let the recipient know who sent the email. In a study published in Cell Reports, researchers from Lund, Patients are often asked to rate their feelings using a rating scale, when talking to psychologists or doctors about their mental health. In a lab environment, i.e., in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumor. The authors said that they chose to study liver cells as Pfizer had reported that about 18% of the vaccine accumulated in the liver in 30 minutes after mice were The CDC says that the COVID-19 vaccines do not change or interact with your DNA in any way, claiming that all of the ingredients in both mRNA and viral vector COVID-19 vaccines (administered in the United States) are discarded from the body once antibodies are produced. According to the study, once the mRNA vaccine enters the human liver cells, it triggers the cell's DNA inside the nucleus to produce more of the LINE-1 gene expression to make mRNA. DETAILS - Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. Clin Microbiol Infect. Intracellular reverse transcription of Pfizer BioNTech COVID-19 mRNA vaccine BNT162b2 in vitro in human liver. The study, conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. CMAJ. No added sugar, full of protein and chocolate! Its WebClaim : Pfizer COVID-19 Vaccine Changed DNA In Liver Cells! In fact. Indeed, about 8 percent of human DNA comes from viruses inserted into our genomes during evolution. Because the study design does not necessarily reflect what happens in most human bodies, he said the findings cannot be extrapolated to make inferences about what would happen in human subjects. 19.8M. Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? Tozinameran (BNT162b2) Vaccine: The Journey from Preclinical Research to Clinical Trials and Authorization. They have two things in common. A segment of the protein called the open reading frame-1, or ORF-1, then goes back into the nucleus, where it attaches to the vaccines mRNA and reverse transcribes into spike DNA. The data represent RNA levels not viral particles, so while the patient samples required equal cycles of PCR to detect RNA, we cant speak to a persons contagiousness because this study does not tell us how much whole virus was present in their nasal passages. This has been the question our study aims to answer. , conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. Giving to Lund The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. Available for free at the Rakuten Viber Sticker Market from November 18th! (AP Photo/Jae C. Hong, File), Connect with the definitive source for global and local news, A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. It has also been shown to affect immune responses. The American Association of Liver Disease has recently published consensus statements for COVID-19 vaccination in subjects with chronic liver disease (CLD). (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 Data, especially data from vaccinated humans, will hopefully sort out the question marks. is the set of instructions to build and sustain a human being. Social media users are falsely claiming that a Swedish study conducted by Lund University found that mRNA COVID-19 vaccines permanently alter human DNA. 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